agarose beads Search Results


nickel agarose beads h 320 5  (Gold Biotechnology Inc)
95
Gold Biotechnology Inc nickel agarose beads h 320 5
Nickel Agarose Beads H 320 5, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/Nickel+Agarose+Beads/pm41999888-133-0-7
Average 95 stars, based on 1 article reviews
nickel agarose beads h 320 5 - by Bioz Stars, 2026-09
95/100 stars
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anti flag magnetic beads  (MedChemExpress)
95
MedChemExpress anti flag magnetic beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Anti Flag Magnetic Beads, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/Anti-Flag+Magnetic+Agarose+Beads/pmc11005161-161-21-24
Average 95 stars, based on 1 article reviews
anti flag magnetic beads - by Bioz Stars, 2026-09
95/100 stars
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anti ddk agarose beads  (OriGene)
92
OriGene anti ddk agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Anti Ddk Agarose Beads, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/Clone+OTI4C5%2C+Anti-DDK+(FLAG)+Monoclonal+Antibody%2C+Agarose+Beads/pm28289210-303-0-3
Average 92 stars, based on 1 article reviews
anti ddk agarose beads - by Bioz Stars, 2026-09
92/100 stars
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chip grade protein g agarose beads  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc chip grade protein g agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Chip Grade Protein G Agarose Beads, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/ChIP-Grade+Protein+G+Agarose+Beads/pmc10900628-344-6-11
Average 93 stars, based on 1 article reviews
chip grade protein g agarose beads - by Bioz Stars, 2026-09
93/100 stars
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protein a g agarose beads  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc protein a g agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Protein A G Agarose Beads, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/Protein+G+Agarose+Beads/10__1096_slash_fj__201801035r-127-0-4
Average 96 stars, based on 1 article reviews
protein a g agarose beads - by Bioz Stars, 2026-09
96/100 stars
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protein a agarose beads  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc protein a agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Protein A Agarose Beads, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/Protein+A+Agarose+Beads/10__1161_slash_circresaha__108__189316-322-1-6
Average 96 stars, based on 1 article reviews
protein a agarose beads - by Bioz Stars, 2026-09
96/100 stars
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ni nta magnetic agarose beads cg  (Qiagen)
96
Qiagen ni nta magnetic agarose beads cg
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Ni Nta Magnetic Agarose Beads Cg, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/Ni-NTA+Magnetic+Agarose+Beads/pm10810159-74-9-28
Average 96 stars, based on 1 article reviews
ni nta magnetic agarose beads cg - by Bioz Stars, 2026-09
96/100 stars
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anti gfp beads  (MedChemExpress)
94
MedChemExpress anti gfp beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Anti Gfp Beads, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/Anti-GFP+Magnetic+Agarose+Beads/bio_rxiv__2025__11__09__687409-327-31-33
Average 94 stars, based on 1 article reviews
anti gfp beads - by Bioz Stars, 2026-09
94/100 stars
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protein g htc agarose beads  (Gold Biotechnology Inc)
94
Gold Biotechnology Inc protein g htc agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Protein G Htc Agarose Beads, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/Protein+G+HTC+Agarose+Beads/us12570720-453-19-24
Average 94 stars, based on 1 article reviews
protein g htc agarose beads - by Bioz Stars, 2026-09
94/100 stars
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q sepharose beads  (Cytiva Europe)
94
Cytiva Europe q sepharose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Q Sepharose Beads, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/Q+Sepharose+Big+Beads/pm25277231-48-14-22
Average 94 stars, based on 1 article reviews
q sepharose beads - by Bioz Stars, 2026-09
94/100 stars
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sp sepharose beads  (Cytiva Europe)
94
Cytiva Europe sp sepharose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Sp Sepharose Beads, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agarose+beads/SP+Sepharose+Big+Beads/10__1128_slash_mcb__00056___20-205-8-11
Average 94 stars, based on 1 article reviews
sp sepharose beads - by Bioz Stars, 2026-09
94/100 stars
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Image Search Results


SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with anti-FLAG-C or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.

Journal: Veterinary Research

Article Title: Occludin and collagen IV degradation mediated by the T9SS effector SspA contributes to blood–brain barrier damage in ducks during Riemerella anatipestifer infection

doi: 10.1186/s13567-024-01304-y

Figure Lengend Snippet: SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with anti-FLAG-C or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.

Article Snippet: The cell lysates were centrifuged at 12 000 rpm for 20 min. Next, the supernatants were incubated with 50 μL of Anti-Flag Magnetic Beads (MedChemExpress, USA) at 4 °C overnight.

Techniques: Expressing, Transfection, Plasmid Preparation, Activity Assay, Recombinant, Immunostaining, Staining, Fluorescence, Immunoprecipitation, Incubation, Magnetic Beads, Western Blot